| Registro completo |
| Provedor de dados: |
Rev. Bras. Ciênc. Avic.
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| País: |
Brazil
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| Título: |
In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens
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| Autores: |
Zimmermann,CEP
Machado,AK
Cadoná,FC
Jaques,JAS
Schlemmer,KB
Lautert,C
Cruz,IBM
Zanette,RA
Leal,DBR
Santurio,JM
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| Data: |
2014-09-01
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| Ano: |
2014
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| Palavras-chave: |
Aflatoxin B1
Broilers
Lymphocytes
PicoGreen
Reactive oxygen species
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| Resumo: |
The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB1 at 48 h (p < 0.05) and of 10 and 20 µg/mL AFB1 at 72 h (p < 0.01 and p < 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p < 0.001). ROS formation significantly increased at 24 h at all concentrations (p < 0.001). The in-vitro results demonstrate that AFB1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300012
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| Editor: |
Fundação APINCO de Ciência e Tecnologia Avícolas
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| Relação: |
10.1590/1516-635x1603307-312
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Poultry Science v.16 n.3 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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